GROWTH KINETICS OF L.PLANTARUM
In our study, we used an MRS broth base and added various sugars as carbon sources to investigate the growth kinetics of Lactobacillus plantarum. Glucose as a carbon source led to the highest metabolic activity and the fastest growth for this strain.
LACTIC ACID BACTERIA IN SOY MILK
We inoculated various lactic acid bacteria in soy milk and monitored their growth and metabolic activity directly in this substrate. Among them, L. brevis exhibited faster growth and higher metabolic activity compared to L. lactis, indicating that L. brevis would be more suitable for soy milk fermentation.
NO SAMPLE IS TOO COMPLEX
Calorimetry, as a non-optical technique, is particularly useful when components like lipids cause the growth media to become turbid. It remains unaffected by opaque and even solid samples, enabling the observation of probiotic activity in complex media that simulates real-world products, providing a more accurate representation of actual conditions. It is also ideal for studying anaerobic bacteria without placing the entire machine inside an anaerobic chamber. Additionally, the batch format enables the checkerboard method to screen for the best combinations and synergies of media components.
Metabolic fingerprints provide insights beyond biomass production. Kinetic growth data can help identify the optimal time to harvest the most robust and stress-tolerant bacteria.
OPTIMIZING PROBIOTIC MEDIA
The expanding species and strains of probiotics demand a wider selection of growth media. The first step in creating the optimal media for probiotic bacteria involves selecting the right components. Each strain of bacteria has preferred carbon and nitrogen sources, which must be considered alongside the goal of large-scale production.
